Exon Level Expression Profiling: a Novel Unbiased Transcriptome Analysis for Body Fluids

ABSTRACT: Transcriptome analysis of partially degraded and fragmented RNA samples from body fluids Global gene expression profiling has shown great promise in high-throughput biomarker discovery for early disease detection in body fluids such as saliva, which is accessible, cost-effective, and non-invasive. However, this goal has not been fully realized because saliva, like many clinical samples, contains partially fragmented and degraded RNAs that are difficult to amplify and detect with prevailing technologies. Here, using nanogram scale salivary RNA as a proof-of-principle example, we describe our progress with a novel poly-A tail independent mRNA amplification strategy combined with the Affymetrix GeneChip Exon arrays. We defined a Salivary Exon Core Transcriptome (SECT) with highly similar exp

ORGANISM(S): Homo sapiens

SUBMITTER: David Wong 

PROVIDER: E-GEOD-7760 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress