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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human thymic T cell subsets T-N, T-25, T-FN and T-R thymocytes

ABSTRACT: This data set is comprised of all thymic T cell subsets presented in this manuscript. These include T-N, T-25, T-FN and T-R thymocytes. Experiment Overall Design: Detailed methods are presented in the manuscript and in the attached Supplementary Information. All RNA samples were derived from cells immediately after isolation from mice. Note that these samples were amplified and labeled with Affymetrix and Enzo reagents, while peripheral T cell samples were amplified and labeled with Ambion reagents.

ORGANISM(S): Mus musculus

SUBMITTER: Alexander Rudensky

PROVIDER: E-GEOD-7770 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Foxp3-dependent programme of regulatory T-cell differentiation.

Gavin Marc A MA Rasmussen Jeffrey P JP Fontenot Jason D JD Vasta Valeria V Manganiello Vincent C VC Beavo Joseph A JA Rudensky Alexander Y AY

Nature 20070114 7129

Regulatory CD4+ T cells (Tr cells), the development of which is critically dependent on X-linked transcription factor Foxp3 (forkhead box P3), prevent self-destructive immune responses. Despite its important role, molecular and functional features conferred by Foxp3 to Tr precursor cells remain unknown. It has been suggested that Foxp3 expression is required for both survival of Tr precursors as well as their inability to produce interleukin (IL)-2 and independently proliferate after T-cell-rece ...[more]

PMID: 17220874

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Transcription profiling of mouse peripheral T cells subsets: T-N, T-25, T-FN and T-R cells; T-25, T-FN and T-R cells after treatment with IL-2; and T-R cells transduced with empty, PDE3B-expressing or PDE3B(H801A)-expressing retroviral vectors

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Transcription profiling of mouse thymic T cells - Foxp3-dependent programme of regulatory T-cell differentiation (GEO Super Series0

Project description:This SuperSeries is composed of the following subset Series:; GSE7280: Gene expression in peripheral cells: effects of Foxp3 and PDE3B; GSE7770: Gene expression in thymic CD4 T cells: effects of Foxp3 Experiment Overall Design: Thymocyte RNA (GSE7770) was processed with Affy and Enzo reagents whereas peripheral cell RNA (GSE7280) was processed with Ambion reagents, which amplified the cRNA more robustly. As a result, the two data sets don't normalize very well to each other and shouldn't be compared directly to each other.

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