Promoter targeted histone acetylation of chromatinized parvoviral genome is essential for infrection progress

ABSTRACT: The association of host histones with parvoviral DNA is poorly understood. We analyzed the chromatinization and histone acetylation of canine parvovirus DNA during infection by confocal imaging and in situ proximity ligation assay combined with chromatin immunoprecipitation and high-throughput sequencing. We found that at late infection parvovirus replication bodies were rich in histones bearing modifications characteristic of transcriptionally active chromatin, i.e. histone H3 lysine acetylation (H3K27ac). The H3K27ac, in particular, was located in close proximity to the viral DNA-binding protein NS1. Importantly, our results show for the first time that in the chromatinized parvoviral genome, particularly the two viral promoters were rich in H3K27ac. Histone acetyltransferase (HAT) inhib

ORGANISM(S): Felis catus

SUBMITTER: Mikko Oittinen 

PROVIDER: E-GEOD-77785 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress