Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Angiogenesis course

ABSTRACT: This is a time course experiment with 4 - 6 slides per time point where all samples were hybridised to time zero. The microarray slides were scanned using a GenePix 4000B Scanner (Axon Instruments, Foster City, CA, USA). The â??SPOTâ?? software package (CSIRO) was used to identify spots and subtract backgrounds. The extracted information was normalised using the â??Statistics in Microarray Analysis" (SMA) open source R package. Scaled print tip group lowes normalisation was performed for each slide and replicate slides were scaled to each other. The cDNAs used in this microarray were identified as part of collaborative project between the IMVS and Bionomics Limited. The project aims were to identify genes up-regulated during in vitro capillary tube formation as targets for angiogenesis-based therapeutics. This SuperSeries is composed of the following subset Series: GSE767: time 0.5 hr to 0 hr GSE771: time 3 hr to 0 hr GSE777: time 6 hr to 0 hr GSE778: time 24 hr to 0 hr Any requests for further information regarding the data generated from this collaboration should be directed to Bionomics Limited (www.bionomics.com.au) at the following address: Bionomics Limited 31 Dalgleish Street Thebarton, South Australia Australia, 5031 Phone: 618 8354 6104 Fax: 618 8354 6199 Email: busdev@bionomics.com.au Refer to individual Series

ORGANISM(S): Homo sapiens

SUBMITTER: S Waterman

PROVIDER: E-GEOD-779 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Expression profiling reveals functionally important genes and coordinately regulated signaling pathway genes during in vitro angiogenesis.

Hahn C N CN Su Z J ZJ Drogemuller C J CJ Tsykin A A Waterman S R SR Brautigan P J PJ Yu S S Kremmidiotis G G Gardner A A Solomon P J PJ Goodall G J GJ Vadas M A MA Gamble J R JR

Physiological genomics 20050419 1

Angiogenesis is a complex multicellular process requiring the orchestration of many events including migration, alignment, proliferation, lumen formation, remodeling, and maturation. Such complexity indicates that not only individual genes but also entire signaling pathways will be crucial in angiogenesis. To define an angiogenic blueprint of regulated genes, we utilized our well-characterized three-dimensional collagen gel model of in vitro angiogenesis, in which the majority of cells synchrono ...[more]

PMID: 15840639

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