Nucleic Acid Programmable Protein Arrays (NAPPA) Somatic p53 Mutations

ABSTRACT: Purpose: Mutations in TP53 induce autoantibody immune responses in a subset of cancer patients, which have been proposed as biomarkers for early detection. Here, we investigate the association of p53 specific autoantibodies with multiple tumor subtypes and determine the association with p53 mutation status and epitope specificity. Experimental Design: IgG p53 autoantibodies (p53-AAb), were quantified in 412 serum saples using a programmable ELISA assay from patients with serous ovarian, pancreatic adenocarcinoma, and breast cancer. To determine if patients generated mutation specific autoantibodies we designed a panel of the most relevant 51 p53 point mutant proteins, to be displayed on custom programmable protein microarrays. To determine the epitope specificity we displayed 12 overlappin

ORGANISM(S): Homo sapiens

SUBMITTER: Benjamin Katchman 

PROVIDER: E-GEOD-77949 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress