Transcription profiling of human monocytes reveals leukotriene D4 induces gene expression through cysteinyl leukotriene type I receptor.

ABSTRACT: Background: Cysteinyl leukotrienes (cysLTs) are important mediators of innate immune responsiveness and chronic inflammatory diseases. CysLTs acting through cysteinyl leukotriene receptors may influence the migration and activity of cells such as eosinophils, monocytes and dendritic cells. Objective: To determine the gene expression signature of human monocytes in response to cysLTs and to elucidate the signaling pathways involved in monocyte activation. Methods: Gene expression was analyzed using oligonucleotide microarrays. Responsiveness to cysLTs was assessed by real-time PCR, calcium flux, kinase activation and chemotaxis assays. Results: Cysteinyl leukotriene type I receptor (CysLTR1) transcript 1 is predominantly expressed in human monocytes and cysLTs signal through CysLTR1 in thes

ORGANISM(S): Homo sapiens

SUBMITTER: Grzegorz Woszczek 

PROVIDER: E-GEOD-7807 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress