Systematic characterization of seminal plasma piRNAs as molecular biomarkers for male infertility

ABSTRACT: Although piwi-interacting RNAs (piRNAs) play pivotal roles in spermatogenesis, little is known about piRNAs in the seminal plasma of infertile males. In this study, we systematically investigated the profiles of seminal plasma piRNAs in infertile males to identify piRNAs that are altered during infertility and evaluate their diagnostic value. Seminal plasma samples were obtained from 211 infertile patients (asthenozoospermia and azoospermia) and 91 fertile controls. High-throughput sequencing technology was employed to screen piRNA profiles in seminal plasma samples pooled from healthy controls and infertile patients. The results identified 61 markedly altered piRNAs in the infertile patient groups compared with the control group. Next, a quantitative RT-PCR assay was conducted in the trai

ORGANISM(S): Homo sapiens

SUBMITTER: Zhen Zhou 

PROVIDER: E-GEOD-78131 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress