Transcription profiling of human donor-matched retinal and choroidal vascular endothelial cells

ABSTRACT: Consistent with clinical observations that posterior uveitis frequently involves the retinal vasculature and recent recognition of vascular heterogeneity, we hypothesized that retinal vascular endothelium was a cell population of unique molecular phenotype. Donor-matched cultures of primary retinal and choroidal endothelial cells from 6 human cadavers were incubated with either Toxoplasma gondii tachyzoites (10:1, parasite:cell) or Escherichia coli lipopolysaccharide (100 ng/mL); control cultures were simultaneously incubated with medium. Gene expression profiling of endothelial cells was performed using oligonucleotide arrays containing probes designed to detect 8747 human transcripts. After normalization, differential gene expression was assessed by the significance analysis of microarra

ORGANISM(S): Homo sapiens

SUBMITTER: Justine Smith 

PROVIDER: E-GEOD-7850 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress