Gene trapping in the Asian malaria vector, Anopheles stephensi

ABSTRACT: A piggyBac transposon-based gene trap element was transformed into the Asian malaria vector, Anopheles stephensi, and remobilized using the jumpstarter approach using genetic crosses. Individuals that displayed a gene trap remobilization event were then photodocumented and their RNA and DNA complements were extracted. The DNA compelement was used to determine the genomic insertion site, while the RNA was used to determine the transcript coverage of the genes into which the transposons inserted. In nearly half of the cases, insertion was identified to fall within introns present in the 5'-UTR of transcripts- which are not indicated in the current ab initio models for Anopheles stephensi. The ability to utilize next generation RNA-Seq elucidated the functionality of the gene trap elements th

ORGANISM(S): Anopheles stephensi

SUBMITTER: William Reid 

PROVIDER: E-GEOD-78771 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress