Induction of cells expressing markers of GABAergic neurons by transcription factors (mRNA)

ABSTRACT: Specific neuronal types derived from embryonic stem cells (ESCs) can facilitate mechanistic studies and potentially aid in regenerative medicine. Existing induction methods, however, mostly rely on the effects of growth factors, which generally tend to result in mixed populations of neurons. Here we report that over-expression of specific transcription factors (TFs) in ESCs can rather guide the differentiation of ESCs towards specific neuron types. Analysis of published data on gene expression changes early (two days) after induction of each of 185 induced TFs implicated candidate TFs for further ESC differentiation studies. After induction for 6 days four of them (Ascl1, Smad7, Nr2f1, and Ascl2) generated a high proportion (>35%) of cells with neural progenitor marker PSA-NCAM and clear

ORGANISM(S): Mus musculus

SUBMITTER: Minoru Ko 

PROVIDER: E-GEOD-78949 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress