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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Yeast cells: Mutant M1 vs. the control with and without oxidative stress

ABSTRACT: Transcription profiles of mutant M1 and the control in the absence/presence of 4mM H2O2 M1 is a mutated Saccharomyces cerevisiae strain. It was obtained through transcriptional engineering of general transcription factor TFIIB. Encoded by SUA7, TFIIB is reported to regulate the expression of over 730 genes in Saccharomyces cerevisiae. M1 showed significant growth improvement compared with the control when challenged with 4mM H2O2. It also had resistance towards high osmotic pressure (1.5M NaCl). Two-condition experiment (no stress and 4mM H2O2 stress): M1 vs the control. Two biological replicates for M1 and the control under each condition.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Hefang Geng

PROVIDER: E-GEOD-79037 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Yeast cells: Mutant M1 vs. the control with and without oxidative stress

Project description:Transcription profiles of mutant M1 and the control in the absence/presence of 4mM H2O2 M1 is a mutated Saccharomyces cerevisiae strain. It was obtained through transcriptional engineering of general transcription factor TFIIB. Encoded by SUA7, TFIIB is reported to regulate the expression of over 730 genes in Saccharomyces cerevisiae. M1 showed significant growth improvement compared with the control when challenged with 4mM H2O2. It also had resistance towards high osmotic pressure (1.5M NaCl).

2016-03-10 | GSE79037 | GEO

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2012-04-23 | E-GEOD-35677 | biostudies-arrayexpress

Expression analysis with Phospholipase C over expression

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2012-09-01 | E-GEOD-35745 | biostudies-arrayexpress

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Project description:In this study, we generate genomic maps of Mediator, Pol II, TBP, TFIIH, TFIIA, TFIIB, TFIIE, TFIIF, by ChIP coupled to next generation sequencing technology (ChIP-seq), in wild type strains from Saccharomyces cerevisiae and in a mutant for the Mediator essential subunit Med10

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Oxidative quality control regulates response to ER stress through translation control

Project description:The transcriptomic response after 3 hours of DTT treatment shows there is not a drastic difference in most genes between WT and skn7â, yap1â . Vma13â, sse2â were also used as part of the study. Organism : Saccharomyces cerevisiae, Agilent Yeast Gene expression 8x15K Array AMADID: 016322

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Gene Expression Analysis in Different Macrophage Phenotypes

Project description:Macrophage activation is the main immunological process occurring during the development of several diseases, and the heterogeneity of macrophage activation or differentiation has been suggested to be involved in disease progression. In the present study, we attempted to identify molecules specifically expressed on human classically activated macrophages (M1) to investigate the significance of the M1-like phenotype in human diseases. Human monocyte-derived macrophages were differentiated into M1, M2a, M2b, and M2c phenotypes, and gene expression profiles were analyzed by cDNA microarray analysis and were used for bioinformatics examination. The gene expression profiles of murine macrophages were additionally evaluated. We identified guanylate-binding protein 5 (GBP5), which is associated with leucine-rich repeat protein 3-mediated inflammasome assembly in the M1 macrophages of both humans and mice. Notably, GBP5 protein expression was detected in cultured M1 macrophages by Western blot analysis. GBP5 is a useful candidate marker of the M1 phenotype. CD14+ monocytes from human PBMC were cultured with GM-CSF(10 ng mLâ1) or M-CSF (50 ng mLâ1) for seven days to differentiate into macrophages.To induce macrophage subtypes [M1, M1(-), M2a, M2b, and M2c], the macrophages were further stimulated for 24 h with LPS (10 ng mLâ1) + IFN-Î³ (50 ng mLâ1), IFN-Î³ (50 ng mLâ1), IL-4 (10 ng mLâ1), IL-1Î² (10 ng mLâ1), and IL-10 (10 ng mLâ1). Control macrophages (M0) were prepared by incubating for 24 h without additional factors.Two independent experiments were performed using different donors.

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