Long‐term engraftment of primary bone marrow stroma promotes hematopoietic reconstitution after transplantation

ABSTRACT: Restoration of hematopoiesis upon bone marrow (BM) transplantation relies on the engraftment, expansion and function of transplanted hematopoietic stem cells (HSC). However, the number of donor‐derived HSC typically remain much below that present in normal individuals and this likely limits and delays hematopoietic recovery. HSC depend on supportive stromal niches, however, whether the pre‐conditioning required for BM transplantation damages this stromal niche has not been evaluated extensively. Using mouse models, we now find that that BM stroma cells (BMSC) are severely and permanently damaged by pre‐conditioning. Transplantation of primary but not cultured BMSC quantitatively reconstitutes stroma function in vivo, which is mediated by a multipotent CD73+ CD105‐ Sca1+ BMSC subpop

ORGANISM(S): Mus musculus

SUBMITTER: Jean-Paul Abbuehl 

PROVIDER: E-GEOD-79091 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress