Cis regulated role of lncRNA in transcriptional or developmental process (ChIRP-Seq)

ABSTRACT: We report that long noncoding RNAs contribute to transcription and developmental process. Thousands of lncRNAs have been identified in the whole genome, and tend to located closely to protein-coding genes. To study position relationship between lncRNA and protein-coding genes, we classified all of lncRNA to several subgroups based on the genome position with their coding neighbors. XH, the head to head subgroup is associated with transcription and development in GO analysis. Here, we knockdown serveral XH lncRNA by shRNA in embryonic stem cells and induce nondirectional differnetiation by removing LIF or neural differnetiation by RA. Knockdown of XH lncRNAs led to uniform downregulation of nearby coding genes, and form regulatory circuits with its nearby coding genes to fine-tune embryonic

ORGANISM(S): Mus musculus

SUBMITTER: Yuyang Lu 

PROVIDER: E-GEOD-79541 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress