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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Mycobacterium tuberculosis and macrophage response

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE6209: The global transcriptional profile of Mycobacterium tuberculosis during human macrophages infection GSE7962: Sigma factor E of Mycobacterium tuberculosis controls the expression of bacterial components that modulate macrophages Keywords: SuperSeries Refer to individual Series

ORGANISM(S): Mycobacterium tuberculosis

SUBMITTER: Virginie Aris

PROVIDER: E-GEOD-7963 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Mycobacterium tuberculosis sigma factor E regulon modulates the host inflammatory response.

Fontán Patricia A PA Aris Virginie V Alvarez María E ME Ghanny Saleena S Cheng Jeff J Soteropoulos Patricia P Trevani Analia A Pine Richard R Smith Issar I

The Journal of infectious diseases 20080901 6

Mycobacterium tuberculosis survives in macrophages and usually subverts the bactericidal mechanisms of these phagocytes. The understanding of this host-pathogen interaction is relevant for the development of new treatments for tuberculosis. The adaptation of M. tuberculosis to intracellular life depends on its ability to regulate the expression of its genes. Sigma factors are important bacterial transcription activators that bind to the RNA polymerase and give it promoter specificity. Sigma fact ...[more]

PMID: 18657035

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Sigma factor E of Mycobacterium tuberculosis controls the expression of bacterial components that modulate macrophages

Project description:Sigma factor E (SigE) controls the expression of genes that are essential for Mtb virulence. In this work, we have identified the SigE regulon during infection of macrophages Our results indicate that SigE regulates the expression of genes involved in the maintenance of Mtb cell envelope integrity and function (i.e., detoxification and secretion). Keywords: strains comparison We compared the global gene expression of the H37Rv strain and the sigE mutant strain of Mtb in different conditions: growing exponentially in liquid media; after 2h of incubation in RPMI; or after 24 hours of infection of human macrophage-like THP-1 cells.

2010-05-25 | E-GEOD-7962 | biostudies-arrayexpress

The global transcriptional profile of Mycobacterium tuberculosis during human macrophages infection

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2010-05-27 | E-GEOD-6209 | biostudies-arrayexpress

The sigma factor E of Mycobacterium tuberculosis modulates the inflammatory response of human THP1 cells

Project description:Mycobacterium tuberculosis (Mtb) is well adapted to survive in macrophages and usually subverts the bactericidal mechanisms of these professional phagocytes. The adaptation of Mtb to the intracellular life depends on its ability to regulate the expression of its genes. Among the most important bacterial transcription activators are the sigma factors that bind to the RNA polymerase and give it promotor specificity. Sigma factor E (SigE) controls the expression of genes that are essential for Mtb virulence. Analysis of the macrophage transcriptional response indicated that proteins encoded by the sigE regulon are involved in the modulation of the macrophage inflammatory response. We compared the global gene expression of THP1 macrophages infected with H37Rv and SigE to the gene expression profile of uninfected macrophages.

2010-05-25 | E-GEOD-7870 | biostudies-arrayexpress

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Project description:Like other bacterial species, Mycobacterium tuberculosis has multiple sigma (s) factors encoded in its genome. In previously published work, we and others have shown that mutations in some of these transcriptional activators render M. tuberculosis sensitive to various environmental stresses and, in some cases, cause attenuated virulence phenotypes. In this paper, we characterize a M. tuberculosis mutant lacking the ECF s factor sigma-H. This mutant was more sensitive than the wild type to heat shock and to various oxidative stresses, but did not show decreased ability to grow inside macrophages. Using quantitative reverse transcription-PCR and microarray technology, we have started to define the sigma-H regulon and its involvement in the global regulation of the response to heat shock and the thiol-specific oxidizing agent diamide. We identified 48 genes whose expression increased after exposure of M. tuberculosis to diamide; out of these, 39 were not induced in the sigH mutant, showing their direct or indirect dependence on sigma-H. Some of these genes encode proteins whose predicted function is related to thiol metabolism, such as thioredoxin, thioredoxin reductase and enzymes involved in cysteine and molybdopterine biosynthesis. Other genes under sigma-H control encode transcriptional regulators such as sigB, sigE, and sigH itself.

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2010-05-25 | E-GEOD-7871 | biostudies-arrayexpress

Transcription profiling of Mycobacterium tuberculosis wild type and sigE mutant strains after exposure to stress induced by SDS

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2007-09-12 | E-TABM-313 | biostudies-arrayexpress

Transcription profiling of mouse lung from a strain overexpressing IL-10 under control of the macrophage-specific CD68 promoter (macIL-10tg mice) after Mycobacterium tuberculosis infection

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2009-01-17 | E-GEOD-14316 | biostudies-arrayexpress

Transcription profiling of Mycobacterium tuberculosis wild type, ideR mutant and ideR complemented mutants strains grown in iron-sufficient or iron-deficient media

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Regulation of the Mycobacterium tuberculosishypoxic response gene encoding alpha-crystallin

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2010-06-05 | E-GEOD-8639 | biostudies-arrayexpress

The Mycobacterium tuberculosis ECF sigma Factor sE: role in global gene expression and survival in macrophages

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