Y. pestis exposed to various concentrations of ciprofloxacin for various time points (treated/nontreated analysis in each array).

ABSTRACT: Cultures of Y. pestis Kimberley53 virulent strain were exposed to different concentrations of ciprofloxacin (including 0.016 µg/ml representing the strains' MIC value) for various time periods. Total RNA samples were extructed and used for cRNA synthesis and labelling (using Cy3-CTP for the treated samples and Cy5-CTP for the non-treated sample in one biological experiment and dye swap for the 2nd replicate of independent biological experiment). 8 Labeled cRNA samples were hybridized to custom made Agilent 8x15K slide (each slide represent 1 time point). Goal: Identifing alterations in gene expression profile which correlates with the ciprofloxacin induced growth inhibition. Those altered mRNA transcrips will be used as a markers for the development of rapid molecular Antibiotic Suscept

ORGANISM(S): Yersinia pestis

SUBMITTER: Ida Steinberger-Levy 

PROVIDER: E-GEOD-79687 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress