CPD-seq mapping of UV damage formation and repair in yeast

ABSTRACT: UV-induced DNA lesions are an important contributor to mutagenesis and cancer, but it is not fully understood how the chromosomal landscape influences UV lesion formation and repair. We have used a novel high-throughput sequencing method to precisely map UV-induced cyclobutane pyrimidine dimers (CPDs) at nucleotide resolution throughout the yeast genome. Analysis of CPD formation reveals that nucleosomal DNA having an inward rotational setting is protected from CPD lesions. In strongly positioned nucleosomes, this nucleosome 'photofootprint' overrides intrinsic dipyrimidine sequence preferences for CPD formation. CPD formation is also inhibited by DNA-bound transcription factors, in effect protecting important DNA elements from UV damage. Analysis of CPD repair revealed a clear signature o

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: John Wyrick 

PROVIDER: E-GEOD-79977 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress