Transcription profiling of mouse large intestine from animals with and without enteric flora

ABSTRACT: Although microbiota plays a critical role for the normal development and function of host immune systems, the detail of the influence, especially on those in the large intestine (LI), remains unknown. Here, we performed transcriptome analysis using Affymetrix GeneChip on LI of germ-free (GF) and specific pathogen free (SPF) mice of IQI, an inbred strain established from ICR. Experiment Overall Design: Male germ-free (GF) and specific pathogen-free (SPF) IQI/Jic mice were bred and maintained in the laboratory of Central Institute for Experimental Animals (CIEA, Kawasaki, Kanagwa, Japan). GF mice were housed in a Trexler-type flexible film isolator in a standard germ free state and screened on a weekly basis for germ-free status by sampling feces sterilely and culturing on MRS-agar plates u

ORGANISM(S): Mus musculus

SUBMITTER: Masahiro Yamamoto 

PROVIDER: E-GEOD-8006 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress