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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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The histone H3K9 demethylase KDM3A promotes anoikis through transcriptional activation of pro-apoptotic genes BNIP3 and BNIP3L

ABSTRACT: We isolated and sequenced the shRNA region of a pSM2 retroviral plasmid to identify which shRNAs were enriched in MCF10A cells grown in detached conditions versus the same MCF10A cells that were grown under normal, attached conditions. Comparison of the ratio of shRNA sequences between cells grown under normal conditions versus cells grown in detached conditions.

ORGANISM(S): synthetic construct

SUBMITTER: Victoria Pedanou

PROVIDER: E-GEOD-80144 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The histone H3K9 demethylase KDM3A promotes anoikis through transcriptional activation of pro-apoptotic genes BNIP3 and BNIP3L

Project description:We isolated and sequenced the shRNA region of a pSM2 retroviral plasmid to identify which shRNAs were enriched in MCF10A cells grown in detached conditions versus the same MCF10A cells that were grown under normal, attached conditions.

2016-07-29 | GSE80144 | GEO

Role of 4EBP1/2 in the response to glucose deprivation

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IL6-STAT3 downstream targets in MCF10A/MCF10A.ErbB2* cells

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Project description:In order to investigate the role of reptin methylation on the expression of hypoxia-responsive genes across the whole genome, we performed a microarray analysis from RNAs isolated from MCF7 cells expressing either control shRNA (shRNA) or reptin shRNA (shreptin) in normoxic and hypoxic conditions. MCF7 cells were transiently transfected with either non-specific shRNA or reptin shRNA in replicates. Cells were grown for 24 hours before being exposed to either normoxic (21% oxygen) or hypoxic (1% oxygen) conditions for 18 hours. Cells were then lysed and RNA was isolated.

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Expression data from KDM3A knockdown MDA-MB-231 cells

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Control shRNA- vs. obscurin shRNA-expressing MCF10A cells

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2009-04-01 | E-MEXP-1353 | biostudies-arrayexpress

Genome-wide analysis of gene expression perturbed by Pontin shRNA in MCF7 subjected to normoxic and hypoxic conditions

Project description:In order to investigate the role of Pontin methylation on the expression of hypoxia responsive genes across the whole genome, we performed a microarray analysis from RNAs isolated from MCF7 cells expressing either control shRNA (shNS) or Pontin shRNA(shPontin) in normoxic and hypoxic conditions. MCF7 cells were transiently transfected with either non-specific shRNA or Pontin shRNA in replicates. Cells were grown for 24 hours before being exposed to either normoxic (21% Oxygen) or hypoxic condition (1% oxygen) for 6,9 hours. Cells were then lysed and RNA isolated.

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