Next Generation Sequencing Compares Effects of microRNA-9 perturbation in control and SZ hiPSC NPCs

ABSTRACT: To follow-up findings that miR-9 was abundantly expressed in control NPCs, significantly down-regulated in a subset of SZ NPCs, and that miR-9 levels/activity, neural migration and diagnosis were strongly correlated, we tested the effect of manipulating miR-9 at cellular, proteomic and transcriptomic levels. Unexpectedly, proteomic- and RNAseq-based analysis revealed that these effects were mediated primarily by small changes in expression of indirect miR-9 targets, rather than large changes in direct miR-9 targets; these indirect targets are enriched for migration-associated genes. Together these data indicate that aberrant levels and activity of miR-9 may be one of the many factors that contribute to SZ risk, at least in a subset of patients. Methods: We compared global transcription of

ORGANISM(S): Homo sapiens

SUBMITTER: Kristen Brennand 

PROVIDER: E-GEOD-80170 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress