Study of RepID/PHIP in DNA Replication inhitiation in Mammalian Cells

ABSTRACT: Mammalian chromosome replication starts from distinct sites, but the principles governing initiation site selection are unclear because proteins essential for DNA replication do not exhibit sequence-specific DNA binding. We identified a replication initiation determinant (RepID) protein that binds a subset of replication initiation sites. A large fraction of RepID binding sites share a common G-rich motif and exhibit elevated replication initiation. RepID is required for initiation of DNA replication from Rep-ID bound replication origins, including the origin at the human beta-globin (HBB) locus. At HBB, RepID is involved in an interaction between the replication origin (Rep-P) and the locus control region. RepID depleted murine embryonic fibroblasts exhibit abnormal replication f

ORGANISM(S): Homo sapiens

SUBMITTER: Christophe Redon 

PROVIDER: E-GEOD-80298 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress