Transcription profiling of human resting and IL2 activated natural killer cells

ABSTRACT: The aim of the study was to investigate the activation of human NK cells by IL2 through analyzing the global gene expression at different time points (0, 2, 8 and 24 hours) after culture with the cytokine IL2 at 100 IU/ml. NK cells with the CD56+/CD16+ and CD3- phenotype were negatively selected by immunomagnetic beads and re-examined by flow-cytometry to ensure greater than 90% purity . Experiment Overall Design: RNA was extracted and pooled from 4 different donors, amplified and labled according to the manufacturer’s instruction (GeneChipU133plus2® , Affymetrix Inc, CA).

ORGANISM(S): Homo sapiens

SUBMITTER: Javeed Iqbal 

PROVIDER: E-GEOD-8059 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress