Regulation of chromatin structure by Set1 H3K4 methyltransferase and Jhd2 H3K4 demethylase [ChIP-seq]

ABSTRACT: Histone H3K4 methylation is connected to gene transcription from yeast to humans, but its mechanistic role in transcription and chromatin dynamics remains poorly understood. Here, we investigated the functions for Set1 and Jhd2, the sole H3K4 methyltransferase and H3K4 demethylase, respectively, in S. cerevisiae. Our data show that Set1 and Jhd2 predominantly co-regulate transcription. We find combined activities of Set1 and Jhd2 via H3K4 methylation contribute to positive or negative transcriptional regulation at shared target genes. Providing mechanistic insights, our data reveal that Set1 and Jhd2 together control nucleosomal occupancy during transcriptional co-regulation. Moreover, we find a remarkable genome-wide co-regulation of nucleosome and chromatin structure by Set1 and Jhd2 at

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Timothy Parnell 

PROVIDER: E-GEOD-81022 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress