Gene expression analysis to identify Runx1 target genes in GMP, MEP and Gene expression signature of Runx1Î?/Î? lin- sca- kit+ CD105- CD16/32+ CD150+ (XMP) progenitors

ABSTRACT: We report the application of single-molecule-based sequencing technology for high-throughput profiling of histone modifications in mammalian cells. By obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of mouse embryonic stem cells, neural progenitor cells and embryonic fibroblasts. We find that lysine 4 and lysine 27 trimethylation effectively discriminates genes that are expressed, poised for expression, or stably repressed, and therefore reflect cell state and lineage potential. Lysine 36 trimethylation marks primary coding and non-coding transcripts, facilitating gene annotation. Trimethylation of lysine 9 and lysine 20 is detected at satellite, telomeric and active long-terminal repeats, and can spread int

ORGANISM(S): Mus musculus

SUBMITTER: Kira Behrens 

PROVIDER: E-GEOD-81181 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress