MiRNA Profile in Three Different Normal Human Ocular Tissues by miRNA-Seq

ABSTRACT: Since microRNAs (miRNAs) have been associated with eye diseases, our study aims to profile ocular miRNA expression in normal human eye tissue using miRNA-Seq in order to provide a foundation for future disease research. Total RNAs were extracted from normal human ciliary body (CB) (n=7), cornea (n=7), and trabecular meshwork (TM) (n=7) samples using mirVana total RNA isolation kit. The sequencing library was prepared using the Illumina TruSeq Small RNA Sample Prep kit and was sequenced using Illumina MiSeq. Generated sequence reads were trimmed and aligned against human reference database using the Bowtie software, and only exact matches to mature miRNAs from miRBase were included. miRTarBase database was used to analyze the gene targets of the miRNAs in our tissues, and expression of a fe

ORGANISM(S): Homo sapiens

SUBMITTER: Yutao Liu 

PROVIDER: E-GEOD-81254 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress