Genomic profiling of microRNA and messenger RNA reveals deregulated microRNA expression in prostate cancer.

ABSTRACT: MicroRNAs are small non-coding RNAs that regulate mRNA function. Recent studies have shown that microRNA expression is altered in tumors. We studied the expression of both microRNAs and mRNAs in 60 primary prostate tumors and 16 non-tumor prostate tissues to evaluate the involvement of microRNAs in prostate cancer. Global microRNA expression was determined in RNA isolated from fresh-frozen human tissues with a custom oligonucleotide microarray chip. Expression analysis of mRNAs using Affymetrix gene chips revealed that Dicer, a key component of microRNA processing, and two microRNA host genes, MCM7 and C9orf5, were significantly up-regulated in prostate tumors. Consistent with the findings, tumors expressed at higher levels the miR-25 cluster (miR-25/miR-93/miR-106b), which maps to intron

ORGANISM(S): Homo sapiens

SUBMITTER: Robert Hudson 

PROVIDER: E-GEOD-8126 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress