Cytoplasmic polyadenylation is a major regulator of mRNA fate during oogenesis and egg activation.

ABSTRACT: The GLD-2 class of poly(A) polymerases regulate the timing of translation of stored transcripts by elongating the poly(A) tails of target mRNAs in the cytoplasm. WISPY is a GLD-2 enzyme that acts in the Drosophila female germline and is required for the completion of the egg-to-embryo transition. Though a handful of WISPY target mRNAs have been identified during both oogenesis and early embryogenesis, we aimed to discover the full range of WISPY targets at each stage. To globally identify these targets, we carried out microarray analysis to look for maternal mRNAs whose poly(A) tails fail to elongate in the absence of WISP function. We examined the polyadenylated portion of the maternal transcriptome in both stage 14 (mature) oocytes and in early embryos that had completed egg activation.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Jeffrey Pleiss 

PROVIDER: E-GEOD-81495 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress