RNAi of human RHAU-depleted HeLa cells reveals The DExH-box RNA helicase RHAU is a nuclear protein involved in transcription and mRNA decay

ABSTRACT: RHAU (RNA helicase-associated with AU-rich element) is a DExH protein that was originally identified as a factor accelerating AU-rich element-mediated mRNA degradation. The finding that RHAU is predominantly localized in the nucleus, despite that mRNA degradation occurs in cytoplasm, prompted us to consider nuclear functions of RHAU. In HeLa cells, RHAU was localized throughout the nucleoplasm with some concentration in nuclear speckles in a manner dependent on ATPase activity. Transcriptional arrest altered its localization to nucleolar caps where it was colocalized with other RNA helicases, p68 and p72, suggesting that RHAU is involved in transcription-related RNA metabolism in the nucleus. To see whether RHAU affects global gene expression either transcriptionally or posttranscriptional

ORGANISM(S): Homo sapiens

SUBMITTER: Fumiko Iwamoto 

PROVIDER: E-GEOD-8192 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress