Domain-Dependent Effects of Insulin and IGF-1 Receptors on Preadipocyte Gene Expression

ABSTRACT: Despite a high degree of homology, insulin and IGF-1 receptors (IR/IGF1R) mediate distinct cellular and physiological functions. Here, using chimeric and site-mutated receptors, we demonstrate how domain differences between IR and IGF1R contribute the distinct functions of these receptors. Receptors with the intracellular domain of IGF1R show increased activation of Shc and Gab-1 and more potent regulation of genes involved in proliferation, corresponding to its higher mitogenic activity. Conversely, receptors with the intracellular domain of IR display higher IRS-1 phosphorylation, stronger regulation of genes in metabolic pathways and more dramatic glycolytic responses to hormonal stimulation. We generated mouse brown preadipocytes in which both insulin and IGF-1 receptors (IR and IGF1R)

ORGANISM(S): Mus musculus

SUBMITTER: Grace Daher 

PROVIDER: E-GEOD-81921 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress