Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptomics response to miR-644 overexpression in three breast cancer cell lines

ABSTRACT: The goal of the study is to investigate the effect of microRNA-644 overexpression by chemical mimics on gene expression in breast cancer cell lines. To this purpose control and microRNA-644 mimics are used to transfect MDA-MB-231 cells, SK-BR-3 cells, and MCF-7 cells respectively. Transcriptomics profiling was performed with Illumina HumanHT-12 V4.0 BeadChip microarray. Three cell lines are treated with scramble microRNA mimic (control herein) and microRNA-644 mimic (miR-644 herein), with two replicates per condition. mRNA expression is compared between miR-644 and control treatment within each cell line, respectively.

ORGANISM(S): Homo sapiens

SUBMITTER: Jitao David Zhang 

PROVIDER: E-GEOD-82058 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The miR-644a/CTBP1/p53 axis suppresses drug resistance by simultaneous inhibition of cell survival and epithelial-mesenchymal transition in breast cancer.

Raza Umar U   Saatci Özge Ö   Uhlmann Stefan S   Ansari Suhail A SA   Eyüpoğlu Erol E   Yurdusev Emre E   Mutlu Merve M   Ersan Pelin Gülizar PG   Altundağ Mustafa Kadri MK   Zhang Jitao David JD   Doğan Hayriye Tatlı HT   Güler Gülnur G   Şahin Özgür Ö  

Oncotarget 20160801 31

Tumor cells develop drug resistance which leads to recurrence and distant metastasis. MicroRNAs are key regulators of tumor pathogenesis; however, little is known whether they can sensitize cells and block metastasis simultaneously. Here, we report miR-644a as a novel inhibitor of both cell survival and EMT whereby acting as pleiotropic therapy-sensitizer in breast cancer. We showed that both miR-644a expression and its gene signature are associated with tumor progression and distant metastasis-  ...[more]