Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture

ABSTRACT: Reliable, scalable and time-efficient culture methods are required to fully realize the clinical and industrial applications of human pluripotent stem (hPS) cells. Here we present a completely defined, xeno-free medium that supports long-term propagation of hPS cells on uncoated tissue-culture plastic. The medium consists of the Essential 8 (E8) formulation supplemented with Inter-α-inhibitor (IαI), a human serum-derived protein, recently demonstrated to activate key pluripotency pathways in mouse PS cells. IαI efficiently induces attachment and long-term growth of both embryonic and induced hPS cell lines when added as a soluble protein to the medium at seeding. IαI-supplementation efficiently supports adaptation of feeder-dependent hPS cells to xeno-free conditions, clonal growth as

ORGANISM(S): Homo sapiens

SUBMITTER: Sara Pijuan-Galitó 

PROVIDER: E-GEOD-82103 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress