The Nrd1-like protein Seb1 coordinates co-transcriptional 3’ end processing and polyadenylation site selection

ABSTRACT: Termination of RNAPII transcription is associated with RNA 3’ end formation. For coding genes, termination is initiated by the cleavage/polyadenylation machinery. In contrast, a majority of noncoding transcription events in S. cerevisiae do not rely on RNA cleavage for termination, but instead terminate via a pathway that requires the Nrd1-Nab3-Sen1 (NNS) complex. Here we show that the S. pombe ortholog of Nrd1, Seb1, does not function in NNS-like termination, but promotes polyadenylation site selection of coding and noncoding genes. We found that Seb1 associates with 3’ end processing factors, is enriched at the 3’ end of genes, and binds RNA motifs downstream of cleavage sites. Importantly, a deficiency in Seb1 resulted in widespread changes in 3’ UTR length as a consequence of i

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Sander Granneman 

PROVIDER: E-GEOD-82326 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress