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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Drosophila heads

ABSTRACT: Profiled the effect of oxidative stress on gene expression in the heads of adult Drosophila. The flies were fed sucrose with 15mM paraquat (experimental condition) or regular sucrose (control condition) for 30 hours, before harvesting at adult d3.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Sige Zou

PROVIDER: E-GEOD-827 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Comparing genomic expression patterns across species identifies shared transcriptional profile in aging.

McCarroll Steven A SA Murphy Coleen T CT Zou Sige S Pletcher Scott D SD Chin Chen-Shan CS Jan Yuh Nung YN Kenyon Cynthia C Bargmann Cornelia I CI Li Hao H

Nature genetics 20040118 2

We developed a method for systematically comparing gene expression patterns across organisms using genome-wide comparative analysis of DNA microarray experiments. We identified analogous gene expression programs comprising shared patterns of regulation across orthologous genes. Biological features of these patterns could be identified as highly conserved subpatterns that correspond to Gene Ontology categories. Here, we demonstrate these methods by analyzing a specific biological process, aging, ...[more]

PMID: 14730301

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Oxidative stess in Drosophila heads

Project description:Profiled the effect of oxidative stress on gene expression in the heads of adult Drosophila. The flies were fed sucrose with 15mM paraquat (experimental condition) or regular sucrose (control condition) for 30 hours, before harvesting at adult d3. Keywords = oxidative stress Keywords = paraquat Keywords: dose response

2004-01-18 | GSE827 | GEO

Differential gene expression profiling in response to paraquat using a Drosophila Parkinson's disease model

Project description:Transcriptomic profiling using Drosophila heads reveals early gene expression responses to transient paraquat exposure. We performed RNAseq profiling of heads from the wild type (WT) Drosophila strain, Canton S (males only), that were fed either 2.5% sucrose (control) or 5 mM PQ in 2.5% sucrose for 12 h.

2019-09-16 | GSE135919 | GEO

Transcription profiling of Drosophila heads heads from adult males, w118 strain

Project description:The w1118 strain is an inbred lab stock widely used in genetic and transgenic studies. w1118 males have a median life span of 35 days. Adult males were collected within 24 hr after eclosion. Approximately 200 flies were maintained in constant darkness in each food bottle at 25Â°C and 70% humidity and were transferred to fresh bottles every 3-4 days. Transcripts were harvested at d3 and d47. To separate the head from the rest of the body, flies were frozen and briefly vortexed in liquid nitrogen. Fly heads were collected using a sieve which retained fly bodies. Total RNA was extracted using Trizol (GIBCO/BRL). Poly(A) RNA was isolated using Oligotex resin (Qiagen). Samples were profiled with Affymetrix DrosGenome1 GeneChips, using standard Affymetrix protocol.

2008-06-16 | E-GEOD-826 | biostudies-arrayexpress

heads only

Project description:heads only

| PRJNA87985 | ENA

Aging in heads from adult Drosophila males, w118 strain

Project description:The w1118 strain is an inbred lab stock widely used in genetic and transgenic studies. w1118 males have a median life span of 35 days. Adult males were collected within 24 hr after eclosion. Approximately 200 flies were maintained in constant darkness in each food bottle at 25°C and 70% humidity and were transferred to fresh bottles every 3-4 days. Transcripts were harvested at d3 and d47. To separate the head from the rest of the body, flies were frozen and briefly vortexed in liquid nitrogen. Fly heads were collected using a sieve which retained fly bodies. Total RNA was extracted using Trizol (GIBCO/BRL). Poly(A) RNA was isolated using Oligotex resin (Qiagen). Samples were profiled with Affymetrix DrosGenome1 GeneChips, using standard Affymetrix protocol. Keywords: time-course

2004-01-18 | GSE826 | GEO

Gel-free mass spectrometry analysis of Drosophila melanogaster heads

Project description:Here we describe a bottom-up approach to analyse an enriched membrane fraction from Drosophila melanogaster heads using multidimensional liquid chromatography (LC) coupled with tandem-mass spectrometry (MS/MS) that relies on a complete solubilisation and digestion of proteins. An enriched membrane fraction was prepared using equilibrium density centrifugation on a discontinual sucrose gradient, followed by solubilisation using FASP method, tryptic and consequential chymotryptic digestion of proteins. Peptides were separated by reversed-phase (RP) LC at high pH in the first dimension and acidic RP-LC coupled directly to Orbitrap Velos Pro mass spectrometer.

2015-07-27 | PXD001712 | Pride

Transcriptome analysis of w1118 and miR-210 mutant adult fly heads

Project description:We report the genome-wide RNA profiling of adult fly heads upon miR-210 mutation

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Transcription profiling of response of adult Drosophila to oxidative and ER stress

Project description:We used oligonucleotide microarrays to address the specificities of transcriptional responses of adult Drosophila to different stresses induced by paraquat and H2O2, two oxidative stressors, and by tunicamycin which induces an endoplasmic reticulum (ER) stress. Flies were tested 24 hours after exposure to continuous stresses induced by ingestion of paraquat, H2O2 or tunicamycin at concentrations leading to similar effects on viability. We used concentrations of 1% H2O2, 5mM paraquat and 12uM of tunicamycin which lead to negligeable mortality at 24 hours. A paraquat concentration of 15mM was also used for comparison with previous studies Both specific and common responses to the three stressors were observed and whole genome functional analysis identified several important classes of stress responsive genes. Within some functional classes, we observed large variabilities of transcriptional changes between isozymes, which may reflect unsuspected functional specificities.

2004-09-30 | E-MEXP-152 | biostudies-arrayexpress

Transcription profiling of Drosophila heads after aging and oxidative stress

Project description:This SuperSeries is composed of the following subset Series:; GSE826: Aging in heads from adult Drosophila males, w118 strain; GSE827: Oxidative stess in Drosophila heads Experiment Overall Design: Refer to individual Series

2008-06-14 | E-GEOD-6430 | biostudies-arrayexpress

In vivo polycystin-1 interactome using heads of Pkd1 knock-in newborn mice

Project description:We generated a new mouse model with three copies of the HA epitope and eGFP knocked-in frame into the endogenous mouse Pkd1 gene by CRISPR/Cas9. The modified allele is fully functional. Using nanobody-coupled beads and large quantities of tissue (8 heads of P1 newborn mice / experiment / condition), we immunoprecipitated a PC1 interactome that was characterized by mass spectrometry.

2023-08-08 | PXD035250 | Pride

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