Loss of MeCP2 in the rat models regression, impaired sociability and transcriptional deficits of Rett syndrome

ABSTRACT: The goal of this study was to characterize a novel Mecp2 allele in the laboratory rat, a distinct rodent species from the laboratory mouse with unique features. The allele was created by zinc finger-nuclease (ZFN) targeting (SAGE/Horizon) of the X-linked gene, Methyl-CpG-Binding Protein 2 (Mecp2), resulting in normal Mecp2 RNA abundance, but absent protein in male rats as expected due to the presence of only one copy of mutant Mecp2, and an approximate 50% reduction in female rats as expected from animals that harbor one wild-type copy and one mutant copy of Mecp2. Behavioral characterization of female rats with the Mecp2 ZFN allele and wild-type littermates was conducted, and Mecp2 ZFN/+ female rats showed behavioral phenotypes that were consistent with disease-like features present durin

ORGANISM(S): Rattus norvegicus

SUBMITTER: Rodney Samaco 

PROVIDER: E-GEOD-83323 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress