MicroRNA expression profiling of xenografted tumors derived from human esophageal squamous cell carcinoma model treated with cisplatin

ABSTRACT: The plasticity of cancer stem cells (CSCs)/tumor-initiating cells (T-ICs) indicates that multiple CSC/T-IC subpopulations exist within a tumor and multiple oncogenic pathways collaborate to maintain the CSC/T-IC state. Here, we aimed to enrich for T-ICs from clinical ESCC tissues. A chemoresistant human esophageal squamous cell carcinoma (ESCC) patient-derived xenograft model was employed to identify miRNA(s) that contribute to ESCC aggressiveness. We used microarrays to demenstrate the microRNA expression underlying different pretreated conditions. NOG mice bearing subcutaneous tumor xenografts derived from clinical ESCC cells were intraperitoneally treated with CDDP or PBS twice weekly for three weeks. Tumor cells were then isolated and re-inoculated subcutaneously into NOG mice for t

ORGANISM(S): Homo sapiens

SUBMITTER: Ai Liu 

PROVIDER: E-GEOD-83362 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress