Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Effect of H2O2 treatment on gene expression of islet derived hybrid cell of 1.1B4

ABSTRACT: We analyzed the comprehensive gene expression changes by H2O2 in pancreatic beta-cell derived 1.1B4 cells using Agilent microarray. The genes of 2903 H2O2 treated were up-regulated and 2283 genes were down-regulated compared with control sample. HMOX1, EGR1, IGF, IL8 and NR4A3 were strongly up-regulated Gene regulation associated with antioxidant defense system in pancreatic beta cells still needs to be elucidated, we analyzed the genes expression with Agilent micro-array after 4 hours of 100mM H2O2 treatment by one color method, and found 2903 genes were up-regulated and 2283 genes were down regulated. Changes of some genes expressions were confirmed by real time PCR.

ORGANISM(S): Homo sapiens

SUBMITTER: Yuzuru Otsuka

PROVIDER: E-GEOD-83369 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:The objective of the study was to figure out the impact of LXR signaling on human macrophages. Primary human macrophages in an early differentiating and a mature differentiation state were treated with the LXR agonist T091317 for 4 and 24 h and transcriptome-wide expression analysis were performed. As LXRa is highly induced during macrophage differentiation and 68% of all LXRs targets change their expression during this process, we suggest that LXRs have essential functions in macrophage development. More than 238 genes are regulated in early as well as mature macrophages by activation of LXRs; most of them are up-regulated. LXRs administrate differentiation state specific as well as common macrophages functions related primarily to lipid homeostasis, immune response and cell fate. Interestingly, almost only genes related to lipid and cholesterol metabolism are overrepresented among early induced genes, whereas genes related to immune functions respond later, implying the existence of indirect mechanisms of control. Furthermore, in early differentiating macrophages cell proliferation and cell death associated genes are induced after 24 h of LXR activation, whereas in mature macrophages, showing high LXRa expression, the same functional cluster respond far earlier (4 h) after LXR ligand treatment. In conclusion, our data suggest that LXRs are modulators of macrophage differentiation, operating principally as positive transcriptional regulators of genes involved in lipid and cholesterol metabolism and by this indirectly influencing the immunophenotype of macrophages. CD14 positive cells of healthy donors were isolated by magnetic separation and differentiated in the presence of human serum to macrophages. At an early differentiating state (16 h after isolation) and at in mature differentiating state (day 11 after isolation) the cells were treated with the synthetical LXR agonist T091317 respective its solvent DMSO for 4 and 24 h. RNA was isolated and a whole genome microarray was performed.

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Effect of H2O2 treatment on gene expression of islet derived hybrid cell of 1.1B4

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