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Transcriptomic analysis of inter- and intra-patient variation in human iPSC cardiomyocytes: Platform for precision medicine to predict drug toxicity

ABSTRACT: In the present study we created and analyzed cardiomyocytes from two separate iPSC clones from the fibroblasts of five different female individuals and two male individuals, using footprint-free Sendai virus RNA-seq of iPSC cardiomyocytes, Ampli-seq of heart left ventricle and iPSC cardiomyocytes (with and without drug treatment) and Exome-seq of patient fibroblasts.

ORGANISM(S): Homo sapiens

SUBMITTER: Joseph Wu

PROVIDER: E-GEOD-83668 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome Profiling of Patient-Specific Human iPSC-Cardiomyocytes Predicts Individual Drug Safety and Efficacy Responses In Vitro.

Matsa Elena E Burridge Paul W PW Yu Kun-Hsing KH Ahrens John H JH Termglinchan Vittavat V Wu Haodi H Liu Chun C Shukla Praveen P Sayed Nazish N Churko Jared M JM Shao Ningyi N Woo Nicole A NA Chao Alexander S AS Gold Joseph D JD Karakikes Ioannis I Snyder Michael P MP Wu Joseph C JC

Cell stem cell 20160818 3

Understanding individual susceptibility to drug-induced cardiotoxicity is key to improving patient safety and preventing drug attrition. Human induced pluripotent stem cells (hiPSCs) enable the study of pharmacological and toxicological responses in patient-specific cardiomyocytes (CMs) and may serve as preclinical platforms for precision medicine. Transcriptome profiling in hiPSC-CMs from seven individuals lacking known cardiovascular disease-associated mutations and in three isogenic human hea ...[more]

PMID: 27545504

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