IMP2 axonal localization, RNA interactome, and function in development of axon trajectories

ABSTRACT: RNA-based regulatory mechanisms play important roles in the development and plasticity of neural circuits and neurologic disease. Developing axons provide a well suited model to study RNA-based regulation, and contain specific subsets of mRNAs that are locally translated and have roles in axon pathfinding. However, the RNA-binding proteins involved in axon pathfinding, and their corresponding mRNA targets, are still largely unknown. Here we find that the RNA-binding protein IMP2 (Igf2bp2) is strikingly enriched in developing axon tracts, including in spinal commissural axons. We used the HITS-CLIP approach to perform a genome-wide identification of RNAs that interact directly with IMP2 in the native context of developing brain. This IMP2 interactome was highly enriched for mRNA targets re

ORGANISM(S): Mus musculus

SUBMITTER: John Flanagan 

PROVIDER: E-GEOD-83822 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress