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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Biological function of antisense RNAs that are synthesized by RNA-dependent RNA polymerases under rehydration

ABSTRACT: To identify RDR1/2/6-dependent antisense RNA loci under 2hr drought stress and 2hr rehydration, expression profiles of Poly (A+) RNA between rdr1/2/6 and wild-type using the custom microarray (GPL19830) were analyzed. rdr1/2/6 and wild-type plants (Arabidopsis thaliana ecotype Columbia) were grown on MS medium for 2 weeks. Then, the plants were transferred to plastic dish and kept for 2hr. Poly (A+) RNA from 100Î¼g total RNA by treating with Ambion Poly A purist twice.5 ng of Poly (A+) RNA were used for the expresion profiling using the custom array (GPL19830).

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Akihiro Matsui

PROVIDER: E-GEOD-83846 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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