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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Equine dendritic cells generated with horse serum have enhanced functionality in comparison to dendritic cells generated with fetal bovine serum

ABSTRACT: EqMoDC generated with horse serum-supplemented medium showed improved morphological characteristics, higher cell viability and exhibited a more robust performance in the functional T cell assays. The study is further described in A. Ziegler, H. Everett, E. Hamza et al., BMC Vet Research, 2016 A two chip study, comparing monocytes, iMoDC and mMoDC derived in vitro from three horses using total RNA extract

ORGANISM(S): Equus caballus

SUBMITTER: Falko Steinbach

PROVIDER: E-GEOD-84031 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Equine dendritic cells generated with horse serum have enhanced functionality in comparison to dendritic cells generated with fetal bovine serum

Project description:EqMoDC generated with horse serum-supplemented medium showed improved morphological characteristics, higher cell viability and exhibited a more robust performance in the functional T cell assays. The study is further described in A. Ziegler, H. Everett, E. Hamza et al., BMC Vet Research, 2016

2016-07-06 | GSE84031 | GEO

Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in horse (miRNA)

Project description:The adaptive response to extreme endurance exercise might involve transcriptional and translational regulation by microRNAs (miRNAs). Therefore, the aim of this study was to define an integrative analysis of blood transcriptome and miRNome in horses before and after a long endurance ride (160 km) using equine microarrays. A total of 2,453 genes and 162 miRNAs were found to be differentially expressed (DEG) between animals at rest and after the endurance ride. To gain understanding of the biological functions regulated by the differentially expressed miRNA, we used a hypergeometric test analysis. Notably, we detected 42 differentially expressed miRNAs that putatively regulate a total of 350 depleted DEGs, involved in glucose metabolism, fatty acid oxidation, mitochondrion biogenesis, and immune response pathways. Graphical Gaussian models in an independent validation set of animals confirmed that 4 miRNAs could be strong candidate regulatory molecules for endurance exercise adaptation. This study represents, to the best of our knowledge, the first integrated comprehensive overview of the miRNA-mRNA co-regulation networks that may play a central role in controlling post-transcriptomic regulations during endurance exercise in horses. Sixty-one Arabian or half-breed Arabian horses (20 females and 41 geldings) aged 10 Â± 2 years (Â±SEM) were recruited on voluntary basis of the owner on three 160 km endurance rides.

2016-09-04 | E-GEOD-73102 | biostudies-arrayexpress

Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in horse (mRNA)

2016-09-04 | E-GEOD-72973 | biostudies-arrayexpress

ChIP-chip from five horse cells with CENP-A

Project description:In the present study we analyzed centromeric localization on chromosome 11 in different horses and results showed that each individual exhibits a different arrangement of CENP-A binding domains.

2015-01-01 | GSE57986 | GEO

Genome-wide detection by array CGH of copy number variations among diverse horse breeds

Project description:Investigating genome-wide characteristics of CNVs in 6 horses representing 6 distinct breeds by using the aCGH method and performed GO and KEGG analysis for the CNVs genes.This result is an important complement to the mapping of horse whole-genome CNVs and helpful to study plateau horses’ adaption to the plateau’s environment.

2014-02-26 | GSE52504 | GEO

New Pathophysiological Insights from Serum Proteome Profiling in Equine Atypical Myopathy.

Project description:Equine atypical myopathy (AM) is a severe environmental intoxication linked to the ingestion of protoxins contained in seeds and seedlings of the sycamore maple (Acer pseudoplatanus) in Europe. The toxic metabolites cause a frequently fatal rhabdomyolysis syndrome in grazing horses. Since toxic metabolites can also be present in co-grazing horses, it is still unclear as to why, in a similar environmental context, some horses show signs of AM whereas others remain clinically healthy. Label-free proteomics analyses on the serum of 26 diseased AM, 23 co-grazers and 11 control horses were performed to provide insights into biological processes and pathways. A total of 43 and 44 differentially expressed proteins between “AM vs co-grazing horses” and “AM vs control horses” were found. Disease-linked changes in the proteome of different groups were found to correlate with detected amounts of toxins and principal component analysis was performed to identify the 29 proteins with the greatest impact on the proteomic differences between groups. Among the pathway-specific changes the glycolysis/gluconeogenesis pathway, the coagulation/complement cascade, and the biosynthesis of amino acid were affected. Sycamore maple poisoning results in a combination of inflammation, oxidative stress, and high energy demand, which is trying to be met by enhanced glycolysis.

2024-02-09 | PXD040429 | Pride

A composite map of copy number variable (CNV) regions in the horse genome and the discovery of CNVs involved in equine XY disorders of sexual development

Project description:38 horses from 16 diverse breeds and Przewalski's Horse were used to generate a composite CNV map of equine genome. This map was used to detect novel copy number variation in six horses affected with disorder of sexual development (DSD).

2014-11-07 | GSE55266 | GEO

Equus caballus breed:American Standardbed horse

Project description:Sequencing American Standardbred horses

| PRJNA495737 | ENA

horse metagenome

Project description:Comparison of fecal microbiota of horses suffering from atypical myopathy and healthy co-grazers.

| PRJNA682516 | ENA

The equine granulocyte proteome: expression changes in autoimmune disease and after stimulation

Project description:We investigated the granulocyte proteome from healthy horses, horses with a spontaneous autoimmune disease (equine recurrent uveitis) and the reaction of granulocytes to different stimuli (IL8, PMA, LPS) on protein level. A total of 2032 proteins describing the whole granulocyte proteome were identified, including 245 proteins (12% of proteome) newly associated to in vivo expression in primary granulocytes (hypothetical proteins). Our studies provide novel information on the granulocyte proteome and contribute to a better understanding of molecular mechanisms involved in activation and recruitment of neutrophils and the role of these cells in modulating and regulating autoimmune diseases. The data presented here is highly relevant for veterinary medicine and has translational quality for spontaneous autoimmune uveitis in man.

2020-01-15 | PXD013648 | Pride

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