Elucidating Combinatorial Chromatin States at Single-Nucleosome Resolution

ABSTRACT: Chromatin Immunoprecipitation followed by sequencing (ChIP-seq) has been instrumental to our current view of chromatin structure and function. It allows genome-wide mapping of histone marks, which demarcate biologically relevant domains. However, ChIP-seq is an ensemble measurement reporting the average occupancy of individual marks in a cell population. Consequently, our understanding of the combinatorial nature of chromatin states relies almost exclusively on correlation between the genomic distributions of individual marks. Here, we report the development of Combinatorial-iChIP to determine the genome-wide co-occurrence of histone marks at single nucleosome resolution. By comparing to null model, we show that certain combinations of overlapping marks (H3K36me3 and H3K79me3) co-occur mo

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Nir Friedman 

PROVIDER: E-GEOD-84240 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress