Selective single cell isolation for genomics using microraft arrays

ABSTRACT: Genomic methods are used increasingly to interrogate the individual cells that compose specific tissues. However, current methods for single cell isolation struggle to phenotypically differentiate specific cells in a heterogeneous population and rely primarily on the use of fluorescent markers. Many cellular phenotypes of interest are too complex to be measured by this approach, making it difficult to connect genotype and phenotype at the level of individual cells. Here we demonstrate that microraft arrays, which are arrays containing thousands of individual cell culture sites, can be used to select single cells based on a variety of phenotypes, such as cell surface markers, cell proliferation and drug response. We then show that a common genomic procedure, RNA-seq, can be readily adapted

ORGANISM(S): Homo sapiens

SUBMITTER: Corbin Jones 

PROVIDER: E-GEOD-85183 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress