Effect pck or ppc expression on intracellular ATP
Ontology highlight
ABSTRACT:
ORGANISM(S): Escherichia coli
SUBMITTER: Pil Kim
PROVIDER: E-GEOD-8532 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
A physiology study of Escherichia coli overexpressing phosphoenolpyruvate carboxykinase.
Kwon Yeong-Deok YD Lee Sang Yup SY Kim Pil P
Bioscience, biotechnology, and biochemistry 20080407 4
To determine whether intracellular ATP levels can be affected, Escherichia coli overexpressing phosphoenolpyruvate carboxykinase (pck) or phosphoenolpyruvate carboxylase (ppc) were grown in glucose minimal medium. The Pck-overexpressing cells showed approximately twice the intracellular ATP concentration, with 22% slower growth than the Ppc-overexpressing strain. This unexpected result of higher ATP coupled with slower growth is discussed based on transcriptome analysis. ...[more]
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