Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of S. cerevisiae response to stress throughout a 15-day wine fermentation

ABSTRACT: We used genome-wide expression analyses to study the response of Saccharomyces cerevisiae to stress throughout a 15-day wine fermentation. Forty percent of the yeast genome significantly changed expression levels to mediate long-term adaptation to an environment in which ethanol is both a stressor and a carbon source. Within this set, we identify a group of 223 genes, designated as the Fermentation Stress Response (FSR), that are dramatically and permanently induced; FSR genes exhibited changes ranging from four-to eighty-fold. The FSR is novel; 62% of the genes involved have not been implicated in global stress responses and 28% of the genes have no functional annotation. Genes involved in respiratory metabolism and gluconeogenesis were expressed during fermentation despite the presence of high concentrations of glucose. Ethanol, rather than nutrient depletion, was responsible for entry of yeast cells into stationary phase. Ethanol seems to regulate yeast metabolism through hitherto undiscovered regulatory networks during wine fermentation. Experiment Overall Design: The main study is based on expression measurements at 0.5,2,3.5,7 and 10 % ethanol (corresponding to roughly 24,48,60,120 and 340 hours). Experiments were done in triplicate (biological replicates). This submission also includes the files at 1 hour and at 12 hours for completeness. (also in triplicate)

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Hennie JJ van Vuuren

PROVIDER: E-GEOD-8536 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Dynamics of the yeast transcriptome during wine fermentation reveals a novel fermentation stress response.

Marks Virginia D VD Ho Sui Shannan J SJ Erasmus Daniel D van der Merwe George K GK Brumm Jochen J Wasserman Wyeth W WW Bryan Jennifer J van Vuuren Hennie J J HJ

FEMS yeast research 20080201 1

In this study, genome-wide expression analyses were used to study the response of Saccharomyces cerevisiae to stress throughout a 15-day wine fermentation. Forty per cent of the yeast genome significantly changed expression levels to mediate long-term adaptation to fermenting grape must. Among the genes that changed expression levels, a group of 223 genes was identified, which was designated as fermentation stress response (FSR) genes that were dramatically induced at various points during ferme ...[more]

PMID: 18215224

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Dataset Information

Transcription profiling of S. cerevisiae response to stress throughout a 15-day wine fermentation

Publications

Dynamics of the yeast transcriptome during wine fermentation reveals a novel fermentation stress response.

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