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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression analysis of CD122HI versus CD122LO VÎ³7+ murine small intestinal IEL at 14-17 days of age

ABSTRACT: Background: Recently have found that Btnl1 expressed by murine enterocytes shapes the local TCR-VÎ³7+ compartment by driving their clonotypic expansion and phenotypic maturation from CD122LO to CD122HI VÎ³7+ cells. The neonatal (D14-17) murine small intestinal epithelium is enriched for VÎ³7+ IEL displaying an â??immatureâ?? CD122LO cell surface phenotype. We believe these cells are precursors for CD122HI VÎ³7+ IEL. Method: To further characterise this putative product-progenitor relationship, CD122hi Vg7+ and CD122lo Vg7+ IEL were purified from individual D14-D17 mice on four independent occasions and compared by total RNAseq. Conclusion: >3000 genes are differentially expressed between CD122HI and CD122LO murine Vg7+ neonatal IEL 4 independent Paired samples of CD122hi Vg7+ and CD122lo Vg7+ IEL were purified from the small intestinal epithelium of pooled 14-17 day old mice. RNA was isolated from sorted samples. Gene expression analysis was performed by total RNAseq.

ORGANISM(S): Mus musculus

SUBMITTER: Rafael Di Marco Barros

PROVIDER: E-GEOD-85422 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression analysis of CD122HI versus CD122LO Vγ7+ murine small intestinal IEL at 14-17 days of age

Project description:Background: The neonatal (D14-17) murine small intestinal epithelium is enriched for Vγ7+ IEL displaying an ‘immature’ CD122LO cell surface phenotype. We believe these cells are precursors for signature CD122HI Vγ7+ IEL that predominate among small intestinal IEL from postnatal D21. Method: To further characterise this potential precursor-product relationship, CD122hi Vg7+ and CD122lo Vg7+ IEL were purified from individual D14-D17 mice on four independent occasions and compared by total RNAseq. Conclusion: ~3000 genes are differentially expressed between CD122HI and CD122LO murine Vg7+ neonatal IEL

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