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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptomic evaluation of bovine embryo quality on blastocysts obtained from peri-pubertal oocyte donors [8 mo vs 14 mo]

ABSTRACT: Assisted reproduction technologies (ART) and high selection pressure observed in the dairy industry are leading towards the use of younger females for reproduction purpose, reducing the interval between generations. This situation might have an impact on embryo quality, which can affect the success rate of the procedures. This study aims to document the effect of donor age on embryo quality, on transcriptomic level, in order to caracterize the effect of using young animals for reproduction purpose. 10 young Holstein cows were used 3 times each at different ages for ovarian stimulation protocols and oocyte collections (at 8, 11 and 14 months). These oocytes were then fertilized in vitro with adult bull semen, generating 3 lots of embryos per animal. Semen from the same bull was used for all females, at all times. Each animal was used as its own control for age-effect evaluation. EmbryoGENE plateform allowed whole genome assessment of gene expression patterns at the blastocyst stage. Embryos from animals at 8 vs 14 months and at 11 vs 14 months were used for microarray hybridization. Validation was done performing RT-qPCR tests on 7 candidate genes.

ORGANISM(S): Bos taurus

SUBMITTER: LÃ©onie Morin-DorÃ©

PROVIDER: E-GEOD-85436 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptomic evaluation of bovine embryo quality on blastocysts obtained from peri-pubertal oocyte donors [11 mo vs 14 mo]

Project description:Assisted reproduction technologies (ART) and high selection pressure observed in the dairy industry are leading towards the use of younger females for reproduction purpose, reducing the interval between generations. This situation might have an impact on 10 young Holstein cows were used 3 times each at different ages for ovarian stimulation protocols and oocyte collections (at 8, 11 and 14 months). These oocytes were then fertilized in vitro with adult bull semen, generating 3 lots of embryos per animal.

2016-08-11 | E-GEOD-85437 | biostudies-arrayexpress

Transcriptomic evaluation of bovine embryo quality on blastocysts obtained from peri-pubertal oocyte donors

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-08-11 | E-GEOD-85438 | biostudies-arrayexpress

Cumulus cell gene expression associated with pre-ovulatory acquisition of developmental competence in bovine oocytes

Project description:This study investigated the cumulus cell (CC) transcriptomic changes during the oocyte developmental competence acquisition period. Six dairy cows were used for 24 oocyte collections and received FSH twice daily over 3 days, followed by FSH withdrawal for 20, 44, 68 and 92 h in four different oestrous cycles for each of the six cows. Half of the cumulusâoocyte complexes were subjected to in vitro maturation, fertilisation and culture to assess blastocyst rate. The other half of the CC underwent microarray analysis and qRT-PCR.

2016-04-01 | E-GEOD-79760 | biostudies-arrayexpress

Differential Gene Expression in Granulosa Cells from Polycystic Ovary Syndrome Patients with and without Insulin Resistance: Identification of Susceptibility Gene Sets through Network Analysis

Project description:Polycystic ovary Syndrome (PCOS) is a heterogeneous endocrine disorder that shows evidence of genetic predidposition among affected individuals. We have utilized the Microarray data from granulosa cells of normal and PCOS women for network construction. Human granulosa cells were isolated from ovarian aspirates from normal and PCOS women undergoing IVF and for each sample, RNA was extracted and hybridized to an Affymetrix GeneChip.

2012-11-06 | E-GEOD-34526 | biostudies-arrayexpress

DNA methylation analysis of bull semen according to peripubertal age (10 months vs. 16 months)

Project description:DNA methylation analysis of bull semen according to peripubertal age (10 months vs. 16 months)

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DNA methylation analysis of bull semen according to peripubertal age (12 months vs. 16 months)

Project description:DNA methylation analysis of bull semen according to peripubertal age (12 months vs. 16 months)

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Methylation pattern analysis of bull semen at 10,12 and 16 months old

Project description:Methylation pattern analysis of bull semen at 10,12 and 16 months old

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Gene expression profile of in vivo and in vitro matured human oocytes revealed by microarray analysis

Project description:Four groups of human oocytes from in vitro fertilization programme were analysed using microarrays: 1.) oocytes matured in vivo (retrieved as mature by ultrasound follicular aspiration but not fertilized after in vitro fertilization), 2.) oocytes matured in vitro in a conventional way (in a maturation medium with added gonadotropins FSH and HCG), 3.) oocytes matured in vitro by a new approach (in a maturation medium with added gonadotropins FSH and HCG, and in a co-culture with cumulus cells from mature oocytes of the same patient), and 4.) not matured oocytes which did not mature in spite of in vitro maturation procedure. Each group of oocytes consisted of three biological replicates with 10 oocytes per replicate therefore 12 oocyte samples and altogether 120 human oocytes were analyzed by microarrays.

2017-03-05 | E-MTAB-5400 | biostudies-arrayexpress

Bull semen

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miRNAs in follicular fluid

Project description:Our aim with this work is to identify and quantify the miRNA profile of the human follicular fluid using a miRNA microarray approach in young and advanced-aged women and in follicles with different oocyte quality. Data collected in this study would be utilized in a future as a marker to predict the oocyte quality. This observational prospective study includes women enrolled in the assisted reproduction program of Hospital Universitari i Politècnic la Fe (Valencia, Spain). The study was accepted by IRB of this Hospital and all couples received informed consent of our study and assisted technique according to service protocols. The study was divided in two stages: Stage 1: Patients (n=21) who had follicles yielding oocytes with different degree of maturation were included (n=21). The follicular fluid samples from 21 patients were pooled in different groups. Expression of miRNA in the follicular fluids was compared within each patient according the degree of oocyte maturation. Stage 2: Patients were allocated into two groups according to the patient's age: advanced age (AA; >36 years old) and young age (YA; <36 years old). Follicular fluids from follicles containing MII oocytes were pooled and miRNA expression was subsequently compared. Please note that the MII samples with underscore (such as MII1 vs MII_1) indicate different pools so they are not the same sample, but we maintained the number because they correspond to paired samples pools for GV and MI.

2015-05-12 | E-GEOD-63737 | biostudies-arrayexpress

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