Gene expression changes in the intestinal crypts of mice with loss of imprinting

ABSTRACT: To identify genes differentially expressed in the intestinal crypts between LOI(+) and LOI(-) mice, we performed microarray experiments using RNA extracted from laser capture microdissection, by microdissecting an average of 8,000 crypts from each of 3 LOI(+) and 3 LOI(-) mice. Keywords: genetic modification design H19 mutant mice with C57BL/6J background carrying a deletion in the H19 gene (3 kb) and 10 kb of the upstream region including differentially methylated region (DMR) were maintained without LOI phenotype by breeding female wild-type C57BL/6J and male H19+/-. Experimental crosses were performed between female H19+/- and male wild-type C57BL/6J to obtain LOI(+) mice and LOI(-) mice. Igf2 on the maternal allele is silenced in LOI(-), i.e. wild type, mice. But when H19 DMR deletion

ORGANISM(S): Mus musculus

SUBMITTER: Patrick Onyango 

PROVIDER: E-GEOD-8583 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress