Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Panton-Valentine Leukocidin Does Not Impact Virulence of Community-associated MRSA

ABSTRACT: Panton-valentine leukocidin (PVL) has been linked to worldwide emergence of community-associated methicillin resistant Staphylococcus aureus (CA-MRSA) -- its role in virulence in unclear. Here we show that PVL had no effect on global gene expression of prominent CA-MRSA strains nor did it affect bacterial clearance from lungs, spleen and kidneys in a highly discriminatory rabbit bacteremia model. These findings negate a large body of epidemiological research that implicated PVL in CA-MRSA virulence. Keywords: mutant vs wild type in 2 different growth phases grown in 2 different medias Wild type USA 300 (strain SF8300), wild type USA 400 (strain MW2) were compared against their respective PVL isogenic knock out strains. Strains were compared at both mid-exponential and stationary phase and grown in both TSB and CCY to determine if PVL plays a role in gene regulation under these conditions.

ORGANISM(S): Staphylococcus aureus

SUBMITTER: Kevin Braughton 

PROVIDER: E-GEOD-8677 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Contribution of Panton-Valentine leukocidin in community-associated methicillin-resistant Staphylococcus aureus pathogenesis.

Diep Binh An BA   Palazzolo-Ballance Amy M AM   Tattevin Pierre P   Basuino Li L   Braughton Kevin R KR   Whitney Adeline R AR   Chen Liang L   Kreiswirth Barry N BN   Otto Michael M   DeLeo Frank R FR   Chambers Henry F HF  

PloS one 20080912 9

Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) strains typically carry genes encoding Panton-Valentine leukocidin (PVL). We used wild-type parental and isogenic PVL-deletion (Delta pvl) strains of USA300 (LAC and SF8300) and USA400 (MW2) to test whether PVL alters global gene regulatory networks and contributes to pathogenesis of bacteremia, a hallmark feature of invasive staphylococcal disease. Microarray and proteomic analyses revealed that PVL does not alter gene o  ...[more]

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