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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse white adipose tissue

ABSTRACT: Trans-10, Cis-12 conjugated linoleic acid (t10c12 CLA) causes fat loss in mouse white adipose tissue (WAT). The early transcriptome changes in WAT were analyzed using high-density microarrays to better characterize the signaling pathways responding to t10c12 CLA. Their gene expression responses between 4 to 24 hr after treatment showed a common set of early gene expression changes indicative of an integrated stress response (ISR). Experiment Overall Design: Mouse WAT RNA for each time point was isolated from two control and two treatment samples for analysis on four microarrays. The two control and two treatment samples consisted of RP tissues from 5 pooled mice for each sample, requiring a total of twenty mice for the four samples taken at each time point.

ORGANISM(S): Mus musculus

SUBMITTER: Jean-Jack Riethoven

PROVIDER: E-GEOD-8679 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Trans-10, cis-12 conjugated linoleic acid activates the integrated stress response pathway in adipocytes.

LaRosa P Christopher PC Riethoven Jean-Jack M JJ Chen Han H Xia Yuannan Y Zhou You Y Chen Mei M Miner Jess J Fromm Michael E ME

Physiological genomics 20070918 3

Trans-10, cis-12 conjugated linoleic acid (t10c12 CLA) causes fat loss in mouse white adipose tissue (WAT) and adipocytes in culture. The early transcriptome changes in treated WAT and 3T3-L1 adipocytes were analyzed using high-density microarrays to better characterize the signaling pathways responding to t10c12 CLA. Gene expression responses between 4 and 24 h after treatment showed a common set of early gene expression changes indicative of an integrated stress response (ISR). The responses o ...[more]

PMID: 17878318

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