Transcription profiling of Drosophila prepupae salivary glands over-expressing Fork head vs. wild type w1118 to identify Fork head-responsive genes in larval salivary glands

ABSTRACT: When misexpressed in late Drosophila prepupae, the transcription factor Fork head (Fkh) blocks death of the larval salivary glands that normally occurs in the early pupa. The aim of the experiment was to identify genes responding to Fkh that might mediate the effect of the protein on cell death and other biological processes. Fkh was expressed in the line P[hs-Fkh111] from a heat-inducible transgene that encodes wild-type Fkh protein. Expression of Fkh was induced by incubating prepupae for 30 min in a 37 °C water bath, starting at 9.5 hours after puparium formation. Salivary glands were dissected at 14 hours after puparium formation and RNA isolated for microarray analysis with Affymetrix GeneChips. Control samples were obtained from w1118 animals treated the same way. The microarray ana

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Michael Lehmann 

PROVIDER: E-GEOD-8722 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress