Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of the human K562 cell line treated with the polyamide-Chl conjugate 1R-Chl

ABSTRACT: To determine whether the polyamide-Chl conjugate 1R-Chl would cause similar changes in global gene expression in K562 cells, affymetrix gene chip analysis was performed using 1R-Chl. Through class comparison analysis, 1R-Chl affected the levels of transcription and genes of interest were determined. Experiment Overall Design: K562 cells were incubated with 1R-Chl (at 250 nM) or in the absence of polyamide, in triplicate for 24 h before RNA purification and microarray analysis at The Scripps Research Institute microarray facility. Affymetrix U133A Plus 2.0 GeneChips were hybridized in groups of three for each of the two groups. The Affymetrix probe set data were imported into BRB Arraytools (3.5.0 Beta 2), selecting the U133 chips used in the experiment and leaving all filters off.

ORGANISM(S): Homo sapiens

SUBMITTER: C James Chou 

PROVIDER: E-GEOD-8832 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Small molecules targeting histone H4 as potential therapeutics for chronic myelogenous leukemia.

Chou C James CJ   Farkas Michelle E ME   Tsai Sherry M SM   Alvarez David D   Dervan Peter B PB   Gottesfeld Joel M JM  

Molecular cancer therapeutics 20080401 4

We recently identified a polyamide-chlorambucil conjugate, 1R-Chl, which alkylates and down-regulates transcription of the human histone H4c gene and inhibits the growth of several cancer cell lines in vitro and in a murine SW620 xenograft model, without apparent animal toxicity. In this study, we analyzed the effects of 1R-Chl in the chronic myelogenous leukemia cell line K562 and identified another polyamide conjugate, 6R-Chl, which targets H4 genes and elicits a similar cellular response. Oth  ...[more]

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